Application

ELISA: 1:5,000-1:50,000

Western Blot: 1:5,000-1:50,000

Optimal working dilutions must be determined by the end user.

Research Sub CategoryWhole Immunoglobulin Secondary Antibodies

Goat anti-Rat IgG Antibody, Alkaline Phosphatase conjugate is an antibody against Rat IgG for use in ELISA & WB.

Research CategorySecondary & Control Antibodies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Highly purified Rat IgG isolated from pooled normal rat serum.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AP140A

Physical form

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge or vortex this product if it is not completely clear after standing for 1-2 hours at room temperature.

Specificity

Reacts with Rat IgG gamma chain, as well as the light chains from all rat immunoglobulin classes. Monospecific on IEP against whole rat serum. Single band on IEP at a concentration of 10 mg/mL when run against rabbit anti goat whole serum.

Storage and Stability

Store lyophilized material at 2-8°C until opened. After reconstitution this product is stable for several weeks at 2-8°C as an undiluted liquid. DO NOT FREEZE. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol and store at 2-8°C for up to 12 months. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after adding glycerol.